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. Author manuscript; available in PMC: 2016 Nov 3.
Published in final edited form as: Structure. 2015 Oct 1;23(11):2099–2110. doi: 10.1016/j.str.2015.08.013

Figure 3. NMR ensembles of complexes of proMMP-7 with DMPC bilayer models showing ordering of some lipids within the fluid bilayers.

Figure 3

(A) Bound to the zwitterionic DMPC bilayer, the proMMP-7 ensemble (cyan) was iteratively fitted to the 1000 best-defined atoms of the enzyme and the 1000 best defined among the lipids, both here and for Figure 2. The grid indicates the average depth of the phosphoryl groups.

(B) The proMMP-7 ensemble (magenta) bound to DMPC bilayers harboring CS, also superposed iteratively, is plotted.

(C) After rotating the view 80°, only DMPC molecul es are plotted from the ensemble containing zwitterionic bilayer. The best defined atoms of the fluid bilayer have full intensity. The shading fades to white where the RMSD of the carbon atoms reaches 2 Å. The nitrogen of the choline groups is blue, oxygen of the phosphoryl groups is red, and the phosphorous orange.

(D) The DMPC molecules of the CS-containing ensemble are plotted in like manner.