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. Author manuscript; available in PMC: 2016 Nov 3.
Published in final edited form as: Structure. 2015 Oct 1;23(11):2099–2110. doi: 10.1016/j.str.2015.08.013

Figure 6. Bicelles alter auto-inhibitory conformation and stimulate activation.

Figure 6

(A) Free proMMP-7 features normal proximity of the catalytic zinc to the conserved cysteine of the auto-inhibitory segment of the pro-domain.

(B) When bound to DMPC/DH6PC bicelles, the conserved auto-inhibitory peptide switches to place the conserved aspartate at the zinc, according to the altered NOE patterns highlighted in Figure S6.

(C) DMPC/DH6PC bicelles (q=0.5) of sufficient concentration accelerate activation (2 h at 37°C) of proMMP-7 (7 μM) to mature MMP-7 (migrating faster by SDS-PAGE).