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. Author manuscript; available in PMC: 2016 Nov 3.
Published in final edited form as: Cell Metab. 2015 Oct 8;22(5):799–810. doi: 10.1016/j.cmet.2015.09.012

Figure 5. cPTH expands Th17 cells, causes bone loss and stimulates bone resorption through activation of G α S in naïve CD4+ cells.

Figure 5

a. cPTH increases the sensitivity to TNF of naïve CD4+ cells from WT and G α S fl/fl mice but not of those from G α SΔCD4,8 mice. Naïve CD4+ cells were sorted from vehicle and cPTH treated mice and cultured with TNF (10–50 ng/ml) to induce their differentiation into Th17 cells. b. TNFR1 mRNA levels in BM CD4+ cells. c. TRAF2 mRNA levels in BM CD4+ cells d. Frequency of BM Th17 cells. e-g. mCT indices of bone volume and structure. h-j. Serum levels of CTX, P1NP and osteocalcin (OCN). Data are shown as mean ± SEM. n = 5 mice per group for panels b-c. n = 16 G α S fl/fl mice per group and 21 G α SΔCD4,8 mice per group for panels d-j. All data passed the Shapiro-Wilk normality test and were analyzed by 2-Way ANOVA. *=p<0.05, **=p<0.01, ***=p<0.001 and ****=p<0.0001 compared to the corresponding vehicle group. # = p<0.05 compared to the G α SΔCD4,8 cPTH group.