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. Author manuscript; available in PMC: 2016 Nov 3.
Published in final edited form as: Cell Metab. 2015 Oct 1;22(5):922–935. doi: 10.1016/j.cmet.2015.09.001

Fig. 4. AMPK substrate prediction using data-independent MS analysis of a global AMPK in vitro kinase assay.

Fig. 4

(A) Immunoblot analysis of known AMPK substrates (ACC and Raptor) in HEK293 lysates subjected to a global AMPK (α1β1γ2) in vitro kinase assay ± active AMPK and the AMPK inhibitor Compound C. (B) – (I) Targeted quantification (mean ± standard deviation, one-way ANOVA corrected for multiple testing, *P < 0.05, **P < 0.01, ***P < 0.005, n=3) of phosphopeptides from HEK293 lysates subjected to a global AMPK (α1β1γ2) in vitro kinase assay using nanoUHPLC-MS/MS on a Q-Exactive MS operated in DIA.