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Proteasomal inhibitors block degradation CTR2 in CTR1 knockout HEK293T cells. (A) Effect of increasing duration of exposure to 100 nM BTZ or CFZ on CTR2 protein level in CTR1 KO-2 cells. (B) Effect of an 8 h exposure to increasing BTZ concentrations on CTR2 level in CTR1 KO-2 cells. (C) Effect of an 8 h exposure to 100 nM BTZ on CTR2 protein level in CTR1 knockout clones. N = 3 for each histogram. Vertical, bars, ± SEM; *, p<0.05; **p<0.01; ***p<0.001.
