Re-expression of CTR1 in CTR1 knockout cells rescues CTR2 in HEK293T and OVCAR8 cells. (A) CTR1 and CTR2 protein levels were measured and quantified in HEK293T wild type (WT), CTR1 KO-1, CTR1 KO-2, CTR1 KO-1mycCTR1 and CTR1 KO-2mycCTR1 cells. N = 3. Vertical, bars, ± SEM; ***, p<0.001. (B) CTR1 and CTR2 protein levels were measured and quantified in OVCAR8, CTR1 KO-1, CTR1 KO-2, CTR1 KO-1mycCTR1, CTR1 KO-2mycCTR1, CTR2 KO-1 and CTR2 KO-2 cells. N = 3. Vertical, bars, ± SEM; *, p<0.05; ***, p<0.001