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. Author manuscript; available in PMC: 2015 Nov 5.
Published in final edited form as: Metallomics. 2015 Jul 24;7(11):1477–1487. doi: 10.1039/c5mt00131e

Table 1.

Genomic sequences of target sites in CTR1 and CTR2 knockout HEK293T and OVCAR8 clones.

CTR1
Allele 1 Allele 2
WT ATGGATCATTCCCACCATATGG
GGATGAGCTATAT		 ATGGATCATTCCCACCATATGG
GGATGAGCTATAT
HEK293T
CTR1 KO-1		 ATGGATCATTCCC - - - - - - - GG
GGATGAGCTATAT(Δ7)		 ATGGATCATTCCCACCATATGG
GGGATGAGCTATAT (+1)
HEK293T
CTR1 KO-2		 ATGGATCATTCCCACCATATGG
GGGATGAGCTATAT (+1)		 ATGGATCATTCCCACCATATGG
GGGATGAGCTATAT (+1)
OVCAR8
CTR1 KO-1		 ATGGATCATTCCCACCATATG-
GGATGAGCTATAT (Δ1)		 ATGGATCATTCCCACCATATG-
GGAT GAGCTATAT (Δ1)
OVCAR8
CTR1 KO-2		 ATGGATCATTCCCACCATATG -
- - - - - - - - - ATAT(Δ10)		 ATGGATCATTCCCACCATATG -
- - - - - - - - ATAT(Δ10)
CTR2
Allele 1 Allele 2
WT CAGCAGACCATCGCAGAGACA
GACGGGGACTCTGCAGGCTCAG
ATTCATTCCC		 CAGCAGACCATCGCAGAGACA
GACGGGGACTCTGCAGGCTCAG
ATTCATTCCC
HEK293T
CTR2 KO-1		 CAGCAGACCA - - - - - - - - - - -
GACGGGGACTCTGCAGGCTCAG
ATTCATTCCC (Δ11)		 CAGCAGACCA - - - - - - - - - - -
GACGGGGACTCTGCAGGCTCAG
ATTCATTCCCT (Δ11)
HEK293T
CTR2 KO-2		 CAGCAGACCATCG- - - - - - - -
- - - - - - - - - - - - - - - - GCTCAG
ATTCATTCCC(Δ24)		 CAGCAGACCATC - CAGAGACA
GACGGGGACTCTGCAGGCTCAG
ATTCATTCCC (Δ 1)
OVCAR8
CTR2 KO-1		 CAGCAG - - - - - - - AGAGACA
GACGGGGACTCTGCAGGCTCAG
ATTCATTCCC (Δ8)		 - - - - - - - - - - - - - CAGAGACA
GACGGGGACTCTGCAGGCTCAG
ATTCATTCCC(Δ13)
OVCAR8
CTR2 KO-2		 CAGCAGACCATCG –AGAGACA
GACGGGGACTCTGCAGGCTCAG
ATTCATTCCC(Δ1)		 CAGCAGACCATCG – AGAGACA
GACGGGGACTCTGCAGGCTCAG
ATTCATTCCC(Δ1)