Table 1.
A comparison of electrophysiological properties of hPSC-CMs, human fetal CMs and human adult CMs.
| Conductio n Velocity |
Action Potentia l Duration |
Action Potential Amplitud e |
Depolarizatio n Velocity (Vmax) |
Resting Membran e Potential |
Maximu m Diastolic Potential |
Capacitanc e |
|
|---|---|---|---|---|---|---|---|
| hPSC-CMs | 1–15 cm/s [1, 2] | 200–500 ms [1–5] | 77–116 mV [3–5] | 6–40 V/s [3–5] 176–201 V/s (long-term culture) [6] |
−37–71 mV [4–6] −66–70 mV (long-term culture) [6] |
−57–75 mV [3] | 10–25 pF [3, 7] |
| Human Fetal CMs | 40–70 cm/s (whole hearts) [8] | 324–416 ms [4] | 20–27 mV [4] | 5–13 V/s [4] | −37–40 mV [4] −82mV (whole hearts) [8] |
−85–86 mV [9] | 16–25 pF [10] |
| Human Adult CMs | 30–100 cm/s [11] | 228–259 ms [9] | 102–110 mV [9] | 254–303 V/s [9] | ~ −90 mV [12] | −82–90 mV [12] | ~200pF [12–14] |
P.W. Burridge, S. Thompson, M.A. Millrod, S. Weinberg, X. Yuan, A. Peters, V. Mahairaki, V.E. Koliatsos, L. Tung, E.T. Zambidis, A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability, PLoS One 6 (2011) e18293.
S.A. Thompson, P.W. Burridge, E.A. Lipke, M. Shamblott, E.T. Zambidis, L. Tung, Engraftment of human embryonic stem cell derived cardiomyocytes improves conduction in an arrhythmogenic in vitro model, J. Mol. Cell. Cardiol. 53 (2012) 15–23.
M.X. Doss, J.M. Di Diego, R.J. Goodrow, Y. Wu, J.M. Cordeiro, V.V. Nesterenko, H. Barajas-Martinez, D. Hu, J. Urrutia, M. Desai, J.A. Treat, A. Sachinidis, C. Antzelevitch, Maximum diastolic potential of human induced pluripotent stem cell-derived cardiomyocytes depends critically on I(Kr), PLoS One 7 (2012) e40288.
C. Mummery, D. Ward-van Oostwaard, P. Doevendans, R. Spijker, S. van den Brink, R. Hassink, M. van der Heyden, T. Opthof, M. Pera, A.B. de la Riviere, R. Passier, L. Tertoolen, Differentiation of human embryonic stem cells to cardiomyocytes: role of coculture with visceral endoderm-like cells, Circulation 107 (2003) 2733–2740.
M.C. Ribeiro, L.G. Tertoolena, J.A. Guadixa, M. Bellina, G. Kosmidisa, C. D’Anielloa, J. Monshouwer-Klootsa, M.J. Goumans, Y.L. Wang, A.W. Feinberg, C.L. Mummery, R. Passiera, Functional maturation of human pluripotent stem cell derived cardiomyocytes in vitro–Correlation between contraction force and electrophysiology, Biomaterials 51 (2015) 138–150.
S.D. Lundy, W.Z. Zhu, M. Regnier, M.A. Laflamme, Structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells, Stem Cells Dev. 22 (2013) 1991–2002.
X. Sheng, M. Reppel, F. Nguemo, F.I. Mohammad, A. Kuzmenkin, J. Hescheler, K. Pfannkuche, Human pluripotent stem cell-derived cardiomyocytes: response to TTX and lidocain reveals strong cell to cell variability, PLoS One 7 (2012) e45963.
G. Gennser, E. Nilsson, Excitation and impulse conduction in the human fetal heart, Acta Physiol. Scand. 79 (1970) 305–320.
I. Koncz, T. Szel, M. Bitay, E. Cerbai, K. Jaeger, F. Fulop, N. Jost, L. Virag, P. Orvos, L. Talosi, A. Kristof, I. Baczko, J.G. Papp, A. Varro, Electrophysiological effects of ivabradine in dog and human cardiac preparations: potential antiarrhythmic actions, Eur. J. Pharmacol. 668 (2011) 419–426.
W.Z. Zhu, L.F. Santana, M.A. Laflamme, Local control of excitation-contraction coupling in human embryonic stem cell-derived cardiomyocytes, PLoS One 4 (2009) e5407.
X. Yang, L. Pabon, C.E. Murry, Engineering adolescence: maturation of human pluripotent stem cell-derived cardiomyocytes, Circ. Res. 114 (2014) 511–523.
E. Drouin, F. Charpentier, C. Gauthier, K. Laurent, H. Le Marec, Electrophysiologic characteristics of cells spanning the left ventricular wall of human heart: evidence for presence of M cells, J. Am. Coll. Cardiol. 26 (1995) 185–192.
W.Z. Zhu, Y. Xie, K.W. Moyes, J.D. Gold, B. Askari, M.A. Laflamme, Neuregulin/ErbB signaling regulates cardiac subtype specification in differentiating human embryonic stem cells, Circ. Res. 107 (2010) 776–786.
S. Polak, K. Fijorek, Inter-individual variability in the pre-clinical drug cardiotoxic safety assessment--analysis of the age-cardiomyocytes electric capacitance dependence, J. Cardiovasc. Transl. Res. 5 (2012) 321–332.