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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Addict Biol. 2015 May 5;21(4):788–801. doi: 10.1111/adb.12256

Figure 2.

Figure 2

CB1 agonism decreases CeA GABA release and chronic ethanol exposure attenuates this effect. A: Representative sIPSCs in control conditions and during WIN 55,212-2 (WIN; 2 µM) application in CeA neurons from naive (top) and CIE (bottom) rats. B: WIN significantly (*p<0.01) decreased the mean sIPSC frequency in CeA neurons of naive rats, but not CIE rats. WIN did not alter the amplitudes, and rise and decay times of sIPSCs in either group. # indicates significant (p<0.01) differences between naive and CIE rats by two-way RM ANOVA. C: Representative mIPSCs in CeA neurons from naive (top) and CIE (bottom) rats. D: WIN significantly (*p<0.01) decreased the mean mIPSC frequency in CeA neurons of naive rats, but not CIE rats. WIN did not alter the amplitudes, and rise and decay times of mIPSCs in either group. #indicates significant (p<0.01) differences between naive and CIE rats by two-way RM ANOVA. E: In naive rats, WIN significantly (*p<0.05) decreased the frequency of sIPSCs. This WIN-induced inhibition of sIPSC frequency is significantly (#p<0.05) reverted (by one way ANOVA) by co-application of the CB1 antagonist, AM251 (2 µM). F: The WIN-induced significant (*p<0.05) inhibition of sIPSC frequency is not altered by co-application of the CB2 antagonist, SR144528 (2 µM). G: In both naive and CIE rats, antagonism of CB1 with AM251 significantly (*p<0.05) increased the sIPSC frequency and blocked the WIN-induced decrease of sIPSC frequency.