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. 2015 Nov 4;35(44):14727–14739. doi: 10.1523/JNEUROSCI.1304-15.2015

Figure 2.

Figure 2.

Dose–response characteristics for DEETGE-CAL-Tat peptide neuroprotection of hippocampal CA1 neurons following GCI. Adult male rats were injected intracebroventrically with various doses of the DEETGE-CAL-Tat peptide at 30 min before GCI and the neuroprotective effect examined in the hippocampal CA1 region at 7 d after GCI. A, Representative hippocampal CA1 sections from sham (Sh), I/R, DEETGE-CAL-Tat (30, 50, 100 μg), or vehicle (Veh; 0.9% NaCl) were subjected to double staining for NeuN (green, indicating surviving cells) and TUNEL (red, indicating apoptotic cells). B, C, Quantification was performed by counting the number of NeuN-positive neurons (B) and TUNEL-positive neurons (C) per 250 μm length in the medial CA1 pyramidal cell layer (n = 5–6). *p < 0.05 compared with Sh group, #p < 0.05 compared with Veh groups. Scale bar, 50 μm. Magnification, 40×.