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. 2015 Nov 4;35(44):14783–14793. doi: 10.1523/JNEUROSCI.2980-15.2015

Figure 1.

Figure 1.

Ischemic injury and leukocyte infiltration are attenuated in CD36−/− mice. A, CD36−/− mice have smaller infarcts than CD36+/+ (WT) 72 h after MCAo; n = 9 or 10 mice/group. *p < 0.001. B, Gating strategy and flow cytometric analysis of infiltrating leukocytes stained for CD45, Ly6C, CD11b, and Ly6G cell surface markers. Six different populations are gated. Infiltrating leukocytes are identified as CD45hi cells and further analyzed for expression of CD11b and Ly6G, identifying CD45hi/CD11bhi/Ly6Glo monocytes/macrophages, CD45hi/CD11bhi/Ly6Ghi neutrophils, CD45hi/CD11blo/Ly6Glo lymphocytes, CD45int/CD11bhi microglia, and CD45lo/CD11blo endothelial cells (EC). C, CD36−/− mice show decreased leukocyte infiltration in the ischemic brain 72 h after MCAo; n = 6 mice/group. *p < 0.05 versus WT. D, A profound decrease of neutrophil infiltration accounts for the reduction in infiltrating leukocytes in the ischemic brain of CD36−/− mice compared with WT mice 72 h after MCAo; n = 6 mice/group. *p < 0.001 versus WT. E, Neutrophil infiltration is reduced more than monocyte/macrophage infiltration in CD36−/− mice relative to WT mice; n = 6/group. Values are mean ± SE. *p < 0.05 versus WT. #p < 0.05 versus neutrophils.