Figure 2.

Effect of CD36 expression in brain cells on ischemic damage and leukocyte infiltration. A, CD36-null mice (KO) transplanted with either WT or KO BM had smaller infarcts than WT mice transplanted with either WT or KO BM 72 h after MCAo (n = 9–11 mice/group). Values are mean ± SE. *p < 0.05 versus WT→WT. B, CD36-null mice (KO) transplanted either with WT or KO BM had less severe neurological deficit than transplanted WT mice with either WT or KO BM 72 h after MCAo (n = 9–11 mice/group). Values are mean ± SE. *p < 0.05 versus WT→WT. C–F, CD36 expression either in the brain or in hematogenous cells facilitates leukocytes (C), neutrophils (D), monocytes/macrophages (E), and lymphocytes (F) to enter the ischemic brain in CD36 chimera mice 72 h after MCAo (n = 9–11 mice/group). While leukocytes and neutrophils are decreased in CD36 KO mice receiving CD36 KO BM compared with WT mice receiving WT marrow, intermediate levels relative to these groups were found in either WT mice receiving CD36 KO BM or CD36 KO mice receiving WT BM. *p < 0.05 versus WT→WT. G, Chimerism in blood leukocytes assayed by genomic qRT-PCR shows a >90% engraftment of KO BM cells in the WT host mice or WT BM cells in the KO host mice (n = 10–15 mice/group). Values are mean ± SE. *p < 0.05 versus WT→WT. H, Percentage of GFP-infiltrating leukocytes associated with vessels or parenchyma in the brain of WT or CD36 KO host brain 72 h after MCAo; n = 6/group. Values are mean ± SE. Right, Infiltrating GFP⁺ leukocytes in neocortex or striatum of WT mice 72 h after MCAo and their association or not with the vascular basement membrane, identified by collagen IV immunocytochemistry. Scale bar, 150 μm.