Model. A, During development, TCPTc growth cones are exposed to 5-HT from raphe nuclei axon projections. 5-HT activates htr2ab in the TCPTc, decreasing expression of ephrinB2a and allowing axons to cross the midline. B, When 5-HT signaling is inhibited by drug, knock-down, or hypoxia, the up-regulation of ephrinB2a expression prevents TCPTc axons crossing the midline.