Figure 3.

REST represses Scg2 directly by binding to RE-1 sequence in the Scg2 promoter. A, The top 25 upregulated and downregulated genes upon REST knockdown in AHPs. * Denotes genes with RE-1 sequences in their promoter regions. B, Chromatin IP analysis shows REST binding to the Scg2 promoter. Primers designed to amplify the RE-1 site in the Scg2 promoter as well as two negative control sites (5 and 2 kb away) were used to assay REST binding. BDNF and Nav1.2 promoter primers were used as positive controls for REST binding. C, Relative amounts of SCG2 protein secreted in the conditioned medium measured by ELISA. **p = 0.0005.