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. 2015 Nov 4;35(44):14922–14942. doi: 10.1523/JNEUROSCI.1897-15.2015

Table 1.

Antibody information

Antigen and antibody name Species/isotype/immunogen Manufacturer information Concentration used
Kv2.1 (L61C/30) Mouse IgG1 mAb binds within aa 595–616 In-house (Trimmer laboratory), RRID:AB_2532100 1:5 tissue culture supernatant
Kv2.1 (K89/34R) Mouse IgG2a mAb binds within aa 837–853 In-house (Trimmer laboratory), recombinant mouse monoclonal antibody expressed in HEK cells RRID: AB_2315768 1:2 tissue culture supernatant
Kv2.1 (K89/34) Mouse IgG1 mAb binds within aa 837–853 NeuroMab catalog #73-014 RRID:AB_10672253 10 μg/ml purified
Kv2.1 (L80/21) Mouse IgG3 mAb binds within aa 837–853 NeuroMab catalog #73-315, RRID:AB_2315862 1:2 tissue culture supernatant
Kv2.2 (N372B/1) Mouse IgG1 mAb binds within aa 764–907 Species reactivity with mouse, rat, ferret, macaque and human NeuroMab catalog #73-369, RRID:AB_2315869 1:5 tissue culture supernatant
Kv2.2 (N372C/51) Mouse IgG1 mAb binds within aa 717–763 NeuroMab catalog #73-358, RRID:AB_2315865 10 μg/ml (LM)
1 μg/ml (EM) purified
Kv2.2 (N372B/60) Mouse IgG2b mAb binds within aa 764–907 species reactivity with mouse and rat NeuroMab catalog #73-360, RRID:AB_2315867 1:5 tissue culture supernatant
Mortalin/Grp 75 (N52A/42) Mouse IgG1 mAb NeuroMab catalog #73-127, RRID:AB_10674108 1:20 tissue culture supernatant
GFP (N86/38) Mouse IgG2a mAb binds within aa 1–238 (full-length) NeuroMab catalog #73-131, RRID:AB_2313651 1:5 tissue culture supernatant
vGlut2 (N29/29) Mouse IgG1 mAb binds within aa 501–582 NeuroMab catalog #75-067, RRID:AB_2239153 1:5 tissue culture supernatant
Cux1 Rabbit pAb, binds within aa 1111–1332 Santa Cruz Biotechnology catalog #sc-13024, RRID:AB_2261231 0.4 μg/ml purified
Ctip2 Rat IgG2a mAb, binds within aa 1–150 Abcam catalog #ab18465, RRID:AB_2064130 0.5 μg/ml purified
Satb2 Mouse IgG1 mAb, binds within C-terminus Abcam catalog #ab51502, RRID:AB_882455 0.1 μg/ml purified