Fig. 1.

Degenerate PCR with optimized primer pairs, gag-PP2-FWD/REV and pro-PP2-FWD/REV. Under the optimized conditions, the gag-PP2-FWD/REV primer pair produced a 420-bp PCR product from mouse genomic DNA (lane 1) but not from the cDNA from infected mouse liver (lane 2) or uninfected mouse liver (lane 3). Similarly, the pro-PP2-FWD/REV primer pair produced a 240-bp PCR product from mouse genomic DNA (lane 5) but not from the cDNA of infected mouse liver (lane 6) or uninfected infected mouse liver (lane 7). Lanes 4 and 8 represent negative controls (no template) for each primer. Lanes 9 and 10 show the RT-PCR products (154 bp) from infected and uninfected mouse liver cDNAs, respectively, using β-actin gene primers. Lane M shows a 100-bp ladder marker, and the lowest band represents 200 bp