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S1P released during blood coagulation binds to albumin. Citrate-plasma or serum pooled from 10 individuals (total volume 500 μL) was subjected to gel filtration chromatography. S1P was extracted from the collected fractions and analysed in LC-MS/MS. ApoM was measured by ELISA and albumin was measured by a commercial kit. Area under the curve was calculated to evaluate the S1P content eluted with either apoM or albumin
