Table 2.
Recovery of S1P in spiked plasma samples. Known amounts of S1P were added to citrate-plasma (n = 6). Experiments were carried out in duplicate with one analysis for each experiment. Recovery was calculated as follows [(final concentration − initial concentration)/added concentration * 100]
| Sample no. | Unspiked (μM) | Spiked (μM, mean ± SD) | Measured difference (μM) | Expected difference (μM) | Recovery (%) |
|---|---|---|---|---|---|
| 1 | 0.628 | 1.26 ± 0.009 | 0.633 | 0.570 | 111 |
| 2 | 0.838 | 1.41 ± 0.028 | 0.572 | 0.570 | 100 |
| 3 | 0.748 | 1.29 ± 0.060 | 0.542 | 0.570 | 95.2 |
| 4 | 0.510 | 1.11 ± 0.046 | 0.602 | 0.570 | 106 |
| 5 | 0.696 | 1.28 ± 0.018 | 0.585 | 0.570 | 103 |
| 6 | 0.838 | 1.42 ± 0.009 | 0.585 | 0.570 | 103 |