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. 2015 Oct 2;5(10):e354. doi: 10.1038/bcj.2015.66

Figure 2.

Figure 2

shRNA-mediated knockdown of IKZF1 or IKZF3 leads to the downregulation of c-Myc and IRF4. Decreased expression of Ikaros (a) or Aiolos (b) in stably transduced MM1.S and U266 MM cells after DOX induction (0.001–1 μg/ml) for 48 h of shRNAs targeting Ikaros (shIKZF1), Aiolos (shIKZF3) or luciferase as control (shLUC). (c) Western blot analysis of Ikaros, Aiolos, c-Myc and IRF4 in MM1.S shIKZF1 or shIKZF3 cells, which were cultured in the absence or the presence of DOX (Dox0.01 μg/ml), for 4 consecutive days (D1–D4). Asterik (*) by Ikaros indicates that only the bottom band was affected using this particular antibody (also see Materials and Methods).