Table 1.

Summary of phenotypic and protein–protein interaction analyses of the inhibitory LUZ24 proteins.

Protein	Growth in Pseudomonas aeruginosa∗		Growth in Escherichia coli∗ MG1655	Prey identification by Y2H	Functional prediction of the Y2H target	α-Gal activity$
	PAO1	PA14
Gp4	+/-	+	+	PA4315	MvaT, transcriptional regulator	23.07 ± 1.46
Gp6	+/-	+/-	+	/
Gp9	-	-	+	PA0298 PA0259	SpuB, glutamylpolyamine synthetase Hypothetical protein	8.04 ± 0.36 Too weak
Gp11	+/-	+/-	+	N/A

^∗Growth after induction: - no growth, +/- less growth, + normal growth, N/A not available. $ The α-galactosidase activity in milliunits per ml per cell. Because the weaker interaction between gp9 and PA0259 falls outside the sensitivity range of the α-galactosidase activity assay, no value could be calculated.