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. 2015 Nov 6;9:140. doi: 10.3389/fnana.2015.00140

FIGURE 2.

FIGURE 2

(A) To obtain a graphic representation of single brain slices the contours of each coronal section surface (external -pial- surface and white/gray matter limits) were drawn: GM, gray matter (gray areas); WM, white matter (white areas); LV lateral ventricle; H, histological analysis (Cresyl violet staining) was performed within some areas, with particular reference to the subcortical gray matter of forebrain regions (see also Figures 4 and 5). (B) Representative drawings showing how single, thick brain slices were cut into blocks (black lines) to be included and frozen for subsequent cryostat cutting; dashed areas indicate the blocks analyzed histologically in these representative slices; all blocks analyzed in this study at the different anterior–posterior levels are listed in Table 4. Hist, histological analysis; Gal, Gallyas method. (C) Comparison of drawings obtained from neonatal Tt and juvenile Sc samples with information available from literature, obtained with different approaches at different ages. a,a’, representative slices of neonatal Tt and juvenile Sc brain material analyzed in this study (see Figure 1); a, coronal brain slices; a’, drawing carried out at the same level; b, originally acquired coronal magnetic resonance images (MRIs) sections of the infant brain (less than 6 months; from Marino et al., 2004a). c, c’, c”, reference images of adult dolphin brains; originally acquired coronal sections stained for cell bodies with Nissl method (c) and for myelinated fibers (c’), from The Yakovlev-Haleem collection at the National Museum of Health and Medicine, Armed Forces Institute of Pathology; c” originally acquired coronal MRI sections of the adult brain (from Marino et al., 2001a – Tursiops truncatus; from Marino et al., 2004b – Stenella longirostris orientalis). (D) Cerebellar tissue samples used in this study. On the left, coronal cutting direction (arrow) to obtain thick slices. In the middle, two representative coronal slices from neonatal Tt and juvenile Sc; scale bars, 1 cm. On the right, cresyl violet staining of cerebellar lamellae; scale bar, 200 μm.