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. 2015 Nov 6;9:432. doi: 10.3389/fncel.2015.00432

Figure 3.

Figure 3

(A) Rn–voltage and tau-voltage relationships are concentration-dependent in the GHK model. Voltage dependence of Rn and tau of 10 (gray) and 30 (orange) mM intracellular Cl− at fixed Erest (−85 mV). Their corresponding K+ and Cl− permeability ratios are 1.34 and 7, respectively. Black: voltage dependence of Rn and tau of 30 intracellular Cl− at a K+/Cl− permeability ratio of 1.34. Ohmic simulation is shown in blue. Each data point was calculated using voltage traces from 15 pA current pulse injection. (B) Voltage responses to current pulses in cerebellar Purkinje neurons are consistent with the GHK model simulation. Left, GHK model simulation using a series of current pulses (−60 to +60 pA at 15 pA interval recorded at −30, 0, and +30 pA holding currents) for 10 and 30 mM intracellular Cl−. Right, voltage responses to current pulses (−30 to +30 pA at 15 pA interval recorded at −30, 0, and +30 pA holding currents) in cerebellar Purkinje neuron. For both simulation and experimental data, gray and orange represent 10 and 30 mM intracellular Cl−. Holding potentials for 10 mM Cl− at −30, 0, +30 pA are identical to values reported in Figure 2E; the holding potentials for 30 mM Cl− are −68, −65, and −60 mV, respectively. (C) Rn–voltage and tau-voltage relationships are concentration-dependent in cerebellar Purkinje neurons. Comparisons of tau and Rn values at each current pulse, between 10 and 30 mM intracellular Cl−. 30 mM Cl− reduced the steepness of the regression slope of Rn-voltage (β10mM = −5.6, n > 6, β30mM = −2.9, n = 6, p = 0.0002).