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In vitro interaction of the rRNA promoter and Histones in B. oleracea. (A) rRNA promoter pulldowns of B. oleracea nuclear extract. Specific bands label with an ∗ represent selective binding proteins to the rDNA promoter. Larger amount was obtained upon PLC treatment to the extract prior to promoter pulldown. (B) GMSA with the rDNA promoter. NE was used in combination with purified histones with PLC pretreatment or without the arrows indicates protein-DNA complexes
