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. 2015 Nov 6;6:976. doi: 10.3389/fpls.2015.00976

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Copyright © 2015 Loza-Muller, Rodríguez-Corona, Sobol, Rodríguez-Zapata, Hozak and Castano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histone H2A methylation in B. oleracea. (A) Purified histones from Brassica as seen in a coomasie stain. M indicate protein weight marker and H the purified histones. The arrow shows the band that is labeled by farwestern in the position of H2A. (B). Western blot of the purified Atfib2. (C) Farwestern of the purified histone fraction. Purified Atfib2 was used to screen the histones and find specific interacting partners. (D) Western blot of H2A to mark the position of this histone after striping the membrane. (E) In vitro methylation assay with or without AtFib2 with the B. oleracea purified histones. (F) Western blot with anti H2A Q105me on histone pulldowns with a control beads (C) or with beads with the rDNA promoter (rDNA). Below is shown the ponceau stain of BSA from the transfer membrane. Numbers indicate the KDA by the marker.