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. 2015 Nov 6;6:976. doi: 10.3389/fpls.2015.00976

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Copyright © 2015 Loza-Muller, Rodríguez-Corona, Sobol, Rodríguez-Zapata, Hozak and Castano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization of methylated H2A in B. oleracea. All cells where stained with DAPI. (A) U2OS cells immunostained with anti H2A Q105me show a specific nucleoli stain. (B–E) Nucleus from B. oleracea fresh cauliflower floral meristem cells with round nucleus. (B) Immunostained with antibodies against fibrillarin. (C) Control secondary antibody only couple to alexa 555. (D) Imunolocalization of methylated H2A. (E) Control secondary antibody only couple to alexa 488.