Table 1. Levels of eicosanoids produced by CYP metabolism in the (a) cortex and (b) subcortical region at 5 and 120 minutes after resuscitation from 9 and 12 minutes of CA.
| Analyte | Sham |
CA (9 minutes) |
CA (12 minutes) |
||
|---|---|---|---|---|---|
| ROSC (minutes) | 5 | 120 | 5 | 120 | |
| (a) Cortex | |||||
| 20-HETE | 3.78±0.41 | 6.79±1.40 | 4.14±0.55 | 12.8±2.78a | 5.35±0.59 |
| 15-HETE | 173±27.2 | 143±19.2 | 291±51.1a | 211±31.5 | 171±61.6 |
| 12-HETE | 1649±366 | 793±61 | 1379±191 | 1732±275 | 1453±478 |
| 8,9-EET | 2.66±0.34 | 39.3±9.32a | 15.9±6.64a | 2.74±0.26 | 2.81±0.56 |
| 11,12-EET | 11.8±4.33 | 20.9±5.2 | 13.9±4.9 | 3.79±0.32 | 5.18±0.87 |
| 14,15-EET | 7.43±2.86 | 44.6±13.3a | 17.8±6.76a | 5.78±1.66 | 3.86±0.62 |
| 5,6-DHETE | 0.63±0.05 | 1.10±0.13 | 0.82±0.07 | 0.84±0.16 | 0.91±0.16 |
| 8,9-DHETE | 0.86±0.08 | 1.31±0.21a | 0.95±0.08 | 1.03±0.15 | 0.81±0.11 |
| 11,12-DHETE | 1.29±0.22 | 2.23±0.57 | 1.18±0.17 | 1.90±0.13 | 1.34±0.44 |
| 14,15-DHETE | 1.41±0.32 | 4.17±0.73a | 1.36±0.34 | 3.39±0.73a | 1.38±0.38 |
| (b) Subcortical region | |||||
| 20-HETE | 4.58±0.87 | 6.34±1.0 | 5.05±0.57 | 7.75±0.79a | 6.30±1.22 |
| 15-HETE | 154±14.3 | 258±39.1 | 375±44a | 144±20.4 | 119±35.3 |
| 12-HETE | 1440±170 | 974±79 | 1263±346 | 1207±173 | 1141±307 |
| 8,9-EET | 2.31±0.51 | 80.3±21.1a | 41.1±7.93a | 3.27±0.61 | 3.05±0.46 |
| 11,12-EET | 20.2±7.71 | 51.4±15.1 | 35.1±6.64 | 4.48±0.87 | 3.66±0.98 |
| 14,15-EET | 14.6±6.01 | 46.5±12.8a | 33.4±5.70a | 4.19±0.74 | 2.53±0.25 |
| 5,6-DHETE | 1.11±0.13 | 1.88±0.30 | 1.33±0.22 | 1.32±0.12 | 1.15±0.13 |
| 8,9-DHETE | 1.59±0.29 | 4.14±0.89a | 3.20±0.51 | 1.18±0.19 | 1.13±0.04 |
| 11,12-DHETE | 1.89±0.55 | 2.94±0.56 | 4.07±0.91 | 1.42±0.25 | 1.32±0.06 |
| 14,15-DHETE | 2.56±0.26 | 2.40±0.24 | 2.79±0.47 | 2.32±0.35 | 1.71±0.54 |
Abbreviations: CA, cardiac arrest; CYP, cytochrome P450; EET, epoxyeicosatrienoic acid; DHETE, dihydroxyeicosatrienoic acid; HET0016, N-hydroxy-N'-(4-n-butyl-2-methylphenyl)formamidine; HETE, hydroxyeicosatetraenoic acid; ROSC, return of spontaneous circulation.
Eicosanoids were quantified in shams and at 5 and 120 minutes after CA, and were reported as pmoL/g tissue. Metabolites from the entire CYP pathway were quantified. Vasoactive metabolites include 20-HETE (vasoconstrictor) and EETs (vasodilators). DHETEs are products of EET degradation by the soluble epoxide hydrolase.
a
P<0.05 versus sham.