Skip to main content
. 2015 Jun 10;35(11):1783–1789. doi: 10.1038/jcbfm.2015.123

Figure 3.

Figure 3

Ketones enhanced mitochondrial complex I activity and reduced protein oxidation. (A) The mitochondrial function kinetics of complex I was represented as MitoXpress fluorescence intensity time series. Mitochondria were isolated from the penumbra. The probe fluorescence intensity indicated oxygen consumption by mitochondria. (B) The amplitude of the time series of complex I was plotted. (C) The mitochondrial function kinetics of complex II was shown. (D) The amplitude of the time series of complex II was plotted. (E) Protein oxidation in the penumbra was analyzed by western blotting. The representative blot of the DNP-derivatized protein (OxyBlot) was shown. β-Actin was used as an internal control. (F) The densitometry quantification of protein levels relative to β-actin was plotted. Data were presented as mean±s.e.m., n=4 per group. **P<0.01 compared with NS group.