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. 2015 Jun 10;35(11):1783–1789. doi: 10.1038/jcbfm.2015.123

Figure 5.

Figure 5

Ketones upregulated Sirtuin 3 (SIRT3), forkhead box O3a (FoxO3a), and superoxide dismutase 2 (SOD2). Homogenates protein was isolated from the penumbra tissue for western blotting. The density was plotted relative to β-actin for (A) SIRT3 and (B) FoxO3a. (C) Representative western blots of SIRT3 and FoxO3a are shown. Mitochondrial protein was isolated from the penumbra tissue for western blotting. The density was plotted relative to VDAC1 for (D) SIRT3 and (E) SOD2. (F) Representative western blots of SIRT3 and SOD2 are shown. Data were presented as mean±s.e.m., n=4 per group. *P<0.05 compared with NS group.