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. 2015 Jun 10;35(11):1783–1789. doi: 10.1038/jcbfm.2015.123

Figure 6.

Figure 6

Sirtuin 3 (SIRT3) mediated the neuroprotective effects of ketones. (A) In primary neuronal cell culture, cells were treated with rotenone at various concentrations. MTT assay for cell viability was plotted with or without ketone treatment. **P<0.01 versus control. ΔΔP<0.01 versus respective rotenone doses. (B) Bioluminescence was measured to represent cellular adenosine triphosphate (ATP) concentration. Neurons were exposed to ketones, nicotinamide or the combination for 48 hours. **P<0.01 versus baseline, ΔΔP<0.01 versus nicotinamide. (C) Primary neuronal cells were transfected by a lentivirus encoding shRNA or SIRT3 cDNA sequence. Cell viability was assessed in cells treated with rotenone, ketones, or both. **P<0.01 versus Vector; ΔΔP<0.01 versus ketones; ##P<0.01 versus Rot 0.1μmol/L+ ketones. Proteins levels of SIRT3 (D), forkhead box O3a (FoxO3a) (E), and superoxide dismutase 2 (SOD2) (F) in neurons treated with ketone or shRNA or the combination were plotted. *P<0.05 versus vector, **P<0.01 versus vector; ΔΔP<0.01 versus ketones. All data were presented as mean±s.e.m., n=8 per group.