Figure 1.

Cloning and sequence alignment of the Der f 28 gene. (A) Cloning and PCR of Der f 28 cDNA (1, DNA marker; 2, Der f 28 gene); (B) restriction enzymatic digestion results of recombinant Hyastatin-pET-44a(+) plasmid (1, DNA marker; 2, Der f 28 DNA); (C) the amino acid sequences of Der f 28.