Figure 5.

Establishment of mouse model of allergic asthma by induction with r-Der f28. (A) The protocol for mouse model of r-Der f 28-induce allergic asthma; (B) airway hyper-responsiveness were measured using the unrestrained whole-body plethysmography. The Penh values were recorded when the mice were stimulated with the increasing concentration of methacholine after the challenge; (C,D) the level of IL-4 and sIgE in Serum were detected by ELISA; (E) the number of eosinophil cell was counted in the BLAF; (F-H) the pathologic changes of pulmonary tissue (hematoxylin-eosin stain, 100×). F, the PBS group; G, the r-Der f 28 group; H, the DME group; ELISA, enzyme-linked immunosorbent assay.