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AA prevented the IL-1β-induced NF-κB activation in cardiomyocytes. The cells were treated with AA (20 µM) for 24 h prior to IL-1β (10 ng/mL) stimulation for 24 h. NF-κB binding activity was determined by EMSA. n=3, ***P<0.001 vs. untreated cells; ##P<0.01 vs. IL-1β-treated cells. IL, interleukin; NF, nuclear factor; AA, asiatic acid; EMSA, electrophoretic mobility shift assay.
