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. 2015 Oct 22;13(5):924–932. doi: 10.1016/j.celrep.2015.09.050

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Serotonergic Axons Are Located in Close Proximity to Processes of pMN-like Adult Progenitor Cells Only Rostral to the Lesion

Spinal cross-sections are shown (dorsal is up; asterisk indicates central canal).

(A and B) Timeline and schematic representations of serotonergic axons in the injured spinal cord in lateral (A) and cross-sectional views (B), indicating the areas of analysis and density of serotonergic axons in relation to pMN-like ERGs.

(C–F) Overviews of 5-HT⁺ labeling in olig2:GFP fish rostral (C and C’) and caudal (E and E’) to the lesion are shown. In the ventro-lateral area, close apposition of serotonergic axons (red) with radial processes (green, arrows) of olig2:GFP⁺ pMN-like progenitor cells (arrowheads) are visible rostral (D), but not caudal (F), to the lesion.

(G and G’) Olig2:dsRed⁺/mbp:GFP⁻ ERGs (arrows), olig2:dsRed⁺/mbp:GFP⁺ oligodendrocytes (arrowheads), and myelin sheaths (open arrowheads) are indicated (G, overview; G’, detail).

(H) PCR after FACS indicates dsRed-only-labeled cells are enriched in the pMN-like ERG fraction, whereas GFP is only amplified in the oligodendrocyte fraction (OLs). EF1alpha is included for comparison. hrt1aa and hrt3a are expressed in adult pMN-like ERGs.

The scale bar in (E’) represents 50 μm for (C), (C’), (E), and (E’); in (F) represents 25 μm for (D) and (F); in (G) represents 25 μm; and in (G’) represents 10 μm. See also Figure S2.