Figure 5.

Collagen Binding by Endo180 D1-4

(A) Solid-phase assay of D1-4-Fc binding to immobilized type I collagen and gelatin (heat-denatured type I collagen). The data shown are representative of five independent experiments carried out in duplicate. For derived K_D values, see the text. Endo180 D1-4-Fc binding to an uncoated control surface is negligible.

(B) SPR analysis of D1-4-Fc (dimer) and D1-4-His (monomer) binding to immobilized type I collagen and gelatin. The data shown are representative of two independent experiments. For derived K_D values, see the text.

Unless indicated otherwise, the experiments were done at pH 7.5. See also Figure S3.