Figure 6.

MSC-IL-37b induced IL-2 expression but repressed IFN-γ expression in splenic CD4⁺ T cells in mice with DSS-induced colitis. On d 10 after DSS administration, mice in each group were euthanized to analyze intracellular cytokine production by splenic CD4⁺ T cells. Splenic cells were analyzed for the expression of the CD4 and CD8 surface markers and the IL-2, IL-4, IL-17, and IFN-γ intracellular markers by flow cytometry. (A) Representative dot plots of IL-2 and IL-4. (B) Representative dot plots of IL-17 and IFN-γ. (C) Frequency of IL-2⁺ cells among splenic CD4⁺ T cells. (D) Frequency of IFN-γ⁺ cells among splenic CD4⁺ T cells. The data are presented as the mean±SEM (n=6 mice per group). ^aP>0.05, ^bP<0.05, ^cP<0.01. The data are representative of three independent experiments.