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. 2015 Oct 12;36(11):1367–1376. doi: 10.1038/aps.2015.76

Figure 4.

Figure 4

Effects of prednisone on B cell subsets in MRL/lpr mice. After treatment with prednisone (2.5 and 5.0 mg/kg) for 13 weeks, the splenic mononuclear cell suspension of each MRL/lpr group was separated from the mouse spleen and analyzed via FC. The column diagram indicates the statistical cell frequency results of the indicated group (n=3), and the pseudo-colored flow cytometry picture represents the cellular distribution of one typical sample from the indicated group. (A) The cell suspension was immunofluorescently stained with an anti-CD19, anti-CD27, and anti-CD38 antibody mix, and the total CD27+CD38+ cells in the CD19+ B cell population were statistically analyzed in the column diagram. A typical cell distribution for each group is shown in the Q2 region of the pseudo-colored flow cytometry picture. (B) The percentages of CD19−CD138+ plasma cells in the model, Pre (2.5 mg/kg), and Pre (5.0 mg/kg) groups were analyzed, and the statistical results are shown in the column diagram. cP<0.01 vs the model group.