Figure 3.
S100A4 was negatively correlated with mineralized nodule formation. The control group (no transfection) exhibited limited calcified deposits in the 12-d osteogenic induction assays (A), whereas the S100A4-RNAi cells formed obvious mineralized nodules (B). However, the overexpression of S100A4 resulted in very few calcified deposits in the culture (C). The OD values for the ARS concentrations indicated significant differences in each of the pairwise comparisons of the 3 (bP<0.05) (D).
