Figure 7. Overlap between charged and polar clusters.

The relatively low overlap indicates that charge-rich regions, termed stalk domains¹⁸, have a low number density of polar residues. The overlap percentage calculation method is explained in the “Methods”, clustering method section. The same analysis was repeated on Disprot proteins as control group (Supplementary Figure S4). Relatively high overlap of polar and charged residues in Disprot shows the significance of the trend observed here.