Figure 4.

(a) Dechlorination profile of TCE and (b) transcript expression of rdhA genes in strain MB. The mbrA transcript was up-regulated 100 fold at 24 h and remained at a similar level after 48 h and decreased tenfold at 72 h corresponding with near complete dechlorination of TCE to trans-DCE. DehaMB_1_0048, DehaMB_1_0051 and DehaMB_1_0159 transcripts were up-regulated at 24 h while DehaMB_5_0010 transcript was expressed at 48 h. However, the transcripts of all four genes were expressed ≥53 fold less than the mbrA transcript at the highest level of expression. All rdhA transcripts, except mbrA, are expressed at less than 1 transcript cell⁻¹ at 72 h. The remaining 27 rdhA genes had expression levels <1.0 transcript cell⁻¹ and are not shown. Error bars, which may be smaller than the symbols, are averages of biological triplicates.