Figure 5.

(a) Dechlorination profile of TCE and (b) transcript expression of vcrA and Deha11a_2_0135 genes in strain 11a. The vcrA transcript was expressed 3.2 fold at 9 h and continued to be expressed another 7.5 fold higher after 24 h, at which TCE was dechlorinated to cis-DCE and VC. The vcrA transcript remained high after complete dechlorination of chloroethenes to ethene after 147 h. Another rdhA transcript (Deha11a_2_0135) showed quantifiable expression at 147 h but only in a single culture out of triplicates. The remaining seven rdhA genes had expression level <1.0 and are not shown. Error bars which may be smaller than the symbols are averages of biological triplicates.