Figure 4. Chronic ABC treatment protects dopaminergic neuronal cell bodies and nerve terminals in 6-OHDA-lesioned rats.

Stereotaxically-lesioned 6-OHDA rats compared to Sham-injected animals. Quantification of the number of dopaminergic neurons in the ipsilateral SNc (a) as visualised in (b) Through immuno-detection of TH (red). (c) [³H]-Mazindol binding in the striatum revealing dopaminergic nerve terminals. 6-OHDA or ABC did not have any effect on the number of TH⁺ neurons and on [³H]-Mazindol binding when the contralateral brain was assessed. Values are mean ± s.e.m. n = 10 per condition, except for [³H]-Mazindol binding where n = 8 for Sham animals and n = 9 for ABC 3. *P < 0.05, ***P < 0.001 versus 6-OHDA; ANOVA with Dunnett’s test. ABC 2: ACP 0.1 mg/kg + BCL 1.5 mg/kg. ABC 3: ACP 0.25 mg/kg + BCL 3.75 mg/kg. Scale bar: 200 μm.