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. 2015 Nov 6;5:16229. doi: 10.1038/srep16229

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Copyright © 2015, Macmillan Publishers Limited

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(a) Western blot and real-time PCR analyses of expression of ZFP36L1 and the monocyte/macrophage differentiation markers CD11B, CD14 and CSF1R. THP-1 cells were infected with lenti-shZFP36L1 and lenti-ctrl respectively followed by PMA induction for 50 h. (b) Expression of the monocytic differentiation marker CD14 was analyzed by flow cytometry in the infected and PMA-induced cells. The red line and the black line indicate untreated cells and CD14 antibody-stained cells respectively. A representative experiment is presented in the top panel and a statistic analysis for three experiments in the bottom. Data are represented as mean ± SD. (c) May-Grünwald Giemsa staining in the infected and PMA-induced cells. The cells were observed under ×40 magnification. The differentiated monocyte/macrophages were indicated with arrows. A representative experiment is presented in the left. A statistical analysis of the differentiated monocyte/macrophages for counting cells in five fields is presented in the right. (d) Western blot and real-time PCR analyses of ZFP36L1 expression and the monocyte/macrophage differentiation markers CD11B, CD14 and CSF1R in THP-1 cells infected with lenti-ZFP36L1 and lenti-ctrl respectively followed by PMA induction for 50 h. (e) Expression of the monocytic differentiation marker CD14 was analyzed by flow cytometry in the lenti-ZFP36L1- or lenti-ctrl-infected and PMA-induced cells. A representative experiment is presented in the top panel and a statistic analysis for three experiments in the bottom. (f) May-Grünwald Giemsa staining in the lenti-ZFP36L1- or lenti-ctrl-infected and PMA-induced cells. The cells were observed under ×40 magnification. A representative experiment is presented in the left and a statistical analysis of the differentiated monocyte/macrophages for counting cells in five fields is presented in the right. *P < 0.05 and **P < 0.01, Student’s t-test.