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. 2015 Nov 6;5:16237. doi: 10.1038/srep16237

Figure 5. The effects of compound combinations on human NPC differentiation.

Figure 5

(A) The effects of compound combinations on the neuronal differentiation of ReNCell VM cells. Cells were treated with 0.5 μm mevastatin; 3 μm kenpaullone; combination; or DMSO control during differentiation. Neurons were stained with DCX antibody. (B) Quantification of the percentage of DCX+ cells with or without treatment. (C) The effects of compound combinations on the dopaminergic neuronal differentiation of ReNCell VM cells. Cells were treated with 3 μM LY364947, 1 μM mevastatin, 3 μM kenpaullone, 1 μM BIO or the combinations of two compounds. Cells were stained with TH antibody. (D) Quantification of the percentage of TH+ cells after the treatments in (C). In all the experiment control received equal volumes of DMSO treatment. *P < 0.05 (red, significantly increased; blue, significantly decreased). Scale bars, 50 μm.