Table 1. Kinetic and equilibrium thermodynamic parameters of interaction of heme exposed human IgG1 Abs with JEV.
| ka × 104 mol−1 s−1 | kd × 10−3 s−1 | KD, nM | ΔH kJ mol−1 | TΔS kJ mol−1 | ΔG kJ mol−1 | |
|---|---|---|---|---|---|---|
| mAb21-JEV E | 1.22 ± 0.10 | 2.93 ± 0.22 | 240 | −2.0 | 35.8 | −37.8 |
| mAb21-JEV EDIII | 1.81 ± 0.04 | 2.58 ± 0.07 | 142 | 6.6 | 45.6 | −39.1 |
| Rtx-JEV E | 1.05 ± 0.01 | 3.52 ± 0.12 | 335 | 16.5 | 53.5 | −37.0 |
| Rtx-JEV EDIII | 0.83 ± 0.01 | 3.63 ± 0.10 | 435 | 4.7 | 41.0 | −36.3 |
Values of the kinetic rate constants (ka and kd) and equilibrium constants (KA and KD) ± SD obtained by global analyses of sensorgrams obtained after injection of heme-exposed mAb21 and Rtx (3.9 to 500 nM) on sensor chip with immobilized JEV E and JEV EDIII. The presented values of the binding kinetics were obtained at 25 °C.