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. 2015 Nov 6;5:16320. doi: 10.1038/srep16320

Figure 2. Mycobacterium tuberculosis PhoP and ESAT-6 help H37Rv inhibit autophagy flux.

Figure 2

H37Ra was complemented with wild type phoP (called as H37Ra:PhoP strain). Panel A shows M1 co-localization coefficient of H37Ra:PhoP with LC3 in the presence or absence of BafA1 treatment, 48 hours post infection. Panel (B) shows relative survival percentage in THP-1 macrophages with respect to H37Rv, H37Ra or H37Ra:PhoP at 48 hours post-infection (mean ± SD, *p-value < 0.05). M1 co-localization coefficient of ∆ESAT-6 mutant of H37Rv with LC3 compartment in BafA1 treated and untreated condition is shown in 2C (mean ± SEM, **p < 0.001). In 2D, relative survival was estimated in H37Rv and ∆ESAT-6 infected THP-1 macrophages, 48 hours post-infection. The ∆ESAT-6 strain infected cells were treated with 3-MA (5 mM, 12 hours) to observe their rescue upon autophagy inhibition (mean ± SD, *p-value < 0.05, **p-value < 0.001).