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. 2015 Nov 6;5:16320. doi: 10.1038/srep16320

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Copyright © 2015, Macmillan Publishers Limited

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Co-localization of H37Ra and H37Rv (green) to LC3 (red) and RAB7 (blue) were examined at 48 hours post-infection. Representative images are shown in panel (A). Panel B shows the plot of Co-localization Coefficient of Mtb to LC3 (Mtb:LC3), Mtb to RAB7 (Mtb:RAB7) and LC3 to RAB7 (LC3:RAB7) at 48 hours post infection (Mean ± SEM, *p < 0.05, **p < 0.001). Panel C shows percentage of Mtb in LC3-RAB7 amphisomes at 48 hours time point (Mean ± SEM, *p-value < 0.05). Panel (D) shows the representative images of H37Ra and H37Rv in amphisomes (white arrow). Scale bar represents 5 μm.