Figure 3. Morphology of PC-12 cells stained with anti-NF-200 antibody.

Cells were incubated with or without NGF (50 ng/ml), hot aqueous extract (25 μg/ml), ethanolic extract (25 μg/ml) or crude polysaccharides (25 μg/ml) for 48 h. Cells in complete F-12 K medium served as a negative control. Cells treated with 50 ng/ml of NGF served as a positive control. Nuclei stained blue and neurofilaments stained green. Scale bars represent 50 μM. Arrows indicate neurite outgrowth.