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. 2015 Nov 6;5:16349. doi: 10.1038/srep16349

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(A) Negative control, cells in complete F-12 K medium only. (B) Positive control, cells treated with 50 ng/ml of NGF. (C) Cells treated with 25 μg/ml of hot aqueous extract. (D–F) Cells were pre-treated with 10 μM U0126 prior to the treatment with NGF or hot aqueous extract. Phosphorylated p44/42 protein stained cell body in green. DAPI stained nuclei in blue. Scale bar = 50 μm.