Figure 2. Hypoxia/re-oxygenation-induced ependymoglia activation and neurogenesis.

(A) Low-magnification images illustrating increased proliferation of ependymoglia cells after re-oxygenation. Arrows point to PCNA⁺/GFAP⁺ cells. (B, C) Quantification of PCNA⁺/GFAP⁺ ependymoglia cells showing increased ependymoglia proliferation after re-oxygenation but not after hypoxia. n = 4–5, *p < 0.05. (Unpaired t-test for B and Mann–Whitney test for C). (D) Low-magnification images illustrating increased number of EdU⁺/Hu⁺ cells in the forebrain parenchyma after re-oxygenation. Arrows point to EdU⁺/Hu⁺ cells. (E) Quantification of EdU⁺/Hu⁺ cells indicating increased neurogenesis in the forebrain parenchyma after re-oxygenation n = 5, *p < 0.05. (Unpaired t-test). Scale bar = 20 μm.

DOI: http://dx.doi.org/10.7554/eLife.08422.006

Figure 2—figure supplement 1. Re-oxygenation leads to increased proliferation assessed by EdU incorporation.

(A) Images illustrating increased EdU incorporation into ependymoglia cells following re-oxygenation. Arrows point to EdU⁺/GFAP⁺ cells. (B) Quantification of the number of EdU⁺/GFAP⁺ cells. n = 4, *p < 0.05. (Unpaired t-test). Scale bar = 20 μm.

DOI: http://dx.doi.org/10.7554/eLife.08422.009