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Expression analysis of MEX67 in the presence of osmolytes [5]. Protein samples were separated by 12% SDS-PAGE and stained with CBB. Lane 1, molecular weight markers. Lane 2, MEX67 un-induced control. Lane 3, MEX67 induced in presence of 100 mM glucose. Lane 4, MEX67 induced in presence of 100 mM sorbitol. Lane 5, MEX67 induced in presence of 100 mM mannitol. Lane 6, MEX67 induced in presence of 1% ethanol. The expression was induced with 0.5 mM IPTG.
